|
KMID : 1098419970050010014
|
|
Korean Journal of Medicinal Crop Science
1997 Volume.5 No. 1 p.14 ~ p.20
|
|
|
Factors Affecting Anther Culture in Schizandra chinensis
|
|
Lee Joong-Ho
Lee Seung-Yeob
|
|
|
Abstract
|
|
|
|
To increase the efficiency of the callus induction in anther culture of Schizandra chinensis, the effects of culture stage, low temperature pretreatment, growth regulators, sucrose and gelling agents were tested on Murashige and Skoog’’s medium. And the effects of ABA and AgNO_3 on organogenesis were investigated. The most suitable stage for anther culture was at the middle to late uninucleate stage, of which the flower size was 6.2{pm}0.6{times}4.0{pm}0.4mm(Length{times}width), and the frequency of callus induction was 13.3%. The effect of low-temperature pretreatment on callus induction was highest as 12.5% in the trearment for 8 days at 4^{circ}C. The combination of 1.0 mg/L 2, 4-D and 0.25 mg/L kinetin for callus induction was most effective as 8.3% among various media. The frequency of callus induction was excellent as 10.8% in 4% sucrose. Effect of gelling agents on callus induction was highest as 9.0% on 0.6% Gelrite medium. The prevention of callus browning was effective on the media supplemented with ABA and AgNO_3 and rooting was promoted on medium supplemented with 5 mg/L ABA. But shoot was not developed.
|
|
|
KEYWORD
|
|
|
Schizandra chinensis, Anther culture, Pretreatment, Growth regulators, Sucrose
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|